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. 2002 Mar;184(5):1444–1448. doi: 10.1128/JB.184.5.1444-1448.2002

TABLE 1.

Conserved charged amino acids located within transmembrane regions of AAHS family members of MFS

Proteina Organism Substrate % Identityc DGXDd Presence of amino acide
R124 R398 E144 R386
PcaK Pseudomonas putida 4-HBA 100 DGLD + + + +
PcaK Pseudomonas aeruginosa 4-HBA 82 DGLD + + + +
PcaK Acinetobacter calcoaceticus 4-HBA 64 DGID + + + +
BenK Acinetobacter calcoaceticus Benzoate 33 DGYD + + + +
FcbT Arthrobacter sp. strain SU 4-Chlorobenzoateb 31 DGMD + + + +
TfdK Ralstonia eutropha 2,4-Dichlorophenoxyacetate 33 DGYD + K + +
MmlH Ralstonia eutropha 4-Methylmuconolactone 25 DSFD + K + +
HppK Rhodococcus globerulus 3-Hydroxyphenylpropionateb 29 DGFE + + + +
MhpT Escherichia coli 3-Hydroxyphenylpropionateb 39 EGLD + + + +
MucK Acinetobacter calcoaceticus cis-cis muconate 20 DGAD + + + +
VanK Acinetobacter calcoaceticus Vanillate 29 DGFD + + + +
a

References or GenBank accession numbers for represented AAHS family members are as follows: P. putida: PcaK, Q51955. P. aeruginosa: PcaK, G83616. A. calcoaceticus: PcaK, Q43975; BenK, AAF63452; MucK, AAC27117; VanK, AAC27108. Arthrobacter sp. strain SU: FcbT, AAD25166. R. eutropha: TfdK, AAC44725; MmlH, CAA67957. R. globerulus: HppK, AAB81315. E. coli: MhpT, P77589.

b

The compound listed is a putative substrate for the respective protein.

c

Percents are relative to 100% amino acid identity for P. putida PcaK based upon GAP analysis (16).

d

DGXD, the four-amino-acid motif located within the first transmembrane region of AAHS family members.

e

Amino acid residue numbers are based upon the P. putida PcaK sequence. +, amino acid residue is conserved in GAP alignments; K, lysine residue at position 398.