FIG. 3.
Expression of NixA mutant proteins. A Western blot of crude membrane extracts of E. coli strains after separation by SDS-PAGE is shown. On the left, the molecular mass of the marker is given in kilodaltons. Lanes: 1, wild type; 2, negative control (nixA deletion mutant); 3, F75A; 4, H79A; 5, N127A; 6, T195A; 7, S197A.