Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2026 Apr 13;16(8):101635. doi: 10.1016/j.jpha.2026.101635

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2026 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 4 — Effect of diosgenin (DG) on the function of high-glucose and high-fat (HGHF)-treated human umbilical vein endothelial cells (HUVECs). Five groups in this part to investigate the effect of DC on HUVECs: control, HGHF, HGHF + low dose of DG (10 μM) (DG^L), HGHF + high dose of DG (20 μM) (DG^H), and HGHF + ferrostatin-1 (Fer-1). (A, B) Evaluation of DG-mediated HUVECs migration (A) and quantitative analysis (B) using the transwell migration assay. (C, D) The scratches assay (C) and quantitative analysis (D) of DG-mediated HUVECs. (E, F) Evaluation of DG-mediated HUVECs angiogenesis (E) and quantitative analysis (F) using the tube formation assay. (G, H) Evaluation of the effect of DG on HUVECs adhesion ability (G) and quantitative analysis (H) using the cell-matrix adhesion assay. (I, J) Western blot (WB) (I) and quantitative analysis (J) of cluster of differentiation 31 (CD31), α-smooth muscle actin (α-SMA), and vascular endothelial growth factor A (VEGF-A) protein expression in HUVECs. Data are presented as the mean ± standard deviation (SD) from 5 independent experiments (n = 5). Statistical significance is denoted as ^∗∗∗P < 0.001.