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Effect of (d)ATP concentration on RecADr-mediated and DNA-dependent (d)ATP hydrolysis. Reactions were carried out as described in Materials and Methods, and reaction mixtures contained 5 μM circular φX174 ssDNA (or 10 μM φX174 linear dsDNA), 2 μM native RecADr protein, 0.5 μM E. coli SSB, and the indicated concentration of ATP (A) or dATP (B).
