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. 2002 Mar;184(6):1649–1660. doi: 10.1128/JB.184.6.1649-1660.2002

FIG. 6.

FIG. 6.

DNA strand exchange promoted by the RecADr protein. Reactions were carried out as described in Materials and Methods, and reaction mixtures contained 6 μM φX174 circular ssDNA, 12 μM φX174 linear dsDNA, 2 μM native RecADr protein, and 0.6 μM E. coli SSB. Reactions also contained 2 mM ATP (or dATP) unless otherwise indicated. Substrate DNA (φX174 linear dsDNA), product (nicked circular dsDNA), and reaction intermediates (joint molecules) are denoted as S, P, and JM, respectively. (A) Comparison of ATP and dATP reactions at pH 7.5. (B) Effects of ATP concentration. Reactions were carried out at pH 8.1. (C) Effects of dATP concentration. Reactions were carried out at pH 8.1. (D) Effect of Mg2+ concentration on DNA strand exchange. Reactions were carried out at pH 7.5. The standard reaction mixture described above and in Materials and Methods was altered by including only the indicated concentration of Mg(acetate)2. (E) Effects of pH on the ATP-dependent DNA strand exchange reaction. The standard reaction was altered by including buffers of the indicated pH. (F) Effects of pH on the dATP-dependent DNA strand exchange reaction. The standard reaction was altered by including buffers of the indicated pHs.