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. 2002 Mar;184(6):1649–1660. doi: 10.1128/JB.184.6.1649-1660.2002

FIG. 7.

FIG. 7.

The RecADr protein binds to dsDNA in preference to ssDNA. (A) Reactions were carried out at pH 7.5 and contained 1.4 μM RecADr protein, DNA as indicated (5 μM css φX174 DNA (ssDNA), 10 μM lds φX174 (Hm dsDNA or dsDNA), and/or 10 μM lds M13mp8 DNA (Ht dsDNA), 0.4 μM E. coli SSB, 2 mM ATP, and an ATP regenerating system. (B) Reactions were carried out at pH 8.1, and reaction mixtures contained 1.0 μM RecADr protein, DNA as indicated [4 μM poly(dT) and/or 8 μM lds M13mp8 DNA], no E. coli SSB, 2 mM dATP, and a dATP regenerating system.