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. 2002 Mar;184(6):1649–1660. doi: 10.1128/JB.184.6.1649-1660.2002

TABLE 1.

Rates of ATP (or dATP) hydrolysisa

Activity and pHs Rate of hydrolysis (μM/min)
dsDNA-dependent During strand exchange ssDNA-dependent
ATP hydrolysis
    pH 7.5 15.5 15.7 11.5
    pH 8.1 3.6 10.2 8.3
dATP hydrolysis
    pH 7.5 16.9 21.5 30
    pH 8.1 20.3 26.9 28
a

Reaction conditions: 5 μM φX circular ssDNA, 10 μM φX linear dsDNA, 2 μM RecADr, 0.5 μM E. coli. SSB, and 2 mM ATP (or dATP).