TABLE 1.
Activity and pHs | Rate of hydrolysis (μM/min)
|
||
---|---|---|---|
dsDNA-dependent | During strand exchange | ssDNA-dependent | |
ATP hydrolysis | |||
pH 7.5 | 15.5 | 15.7 | 11.5 |
pH 8.1 | 3.6 | 10.2 | 8.3 |
dATP hydrolysis | |||
pH 7.5 | 16.9 | 21.5 | 30 |
pH 8.1 | 20.3 | 26.9 | 28 |
Reaction conditions: 5 μM φX circular ssDNA, 10 μM φX linear dsDNA, 2 μM RecADr, 0.5 μM E. coli. SSB, and 2 mM ATP (or dATP).