Transcriptional induction of antioxidant genes in response to selenate and selenite. Total cellular RNA was isolated from cells treated with SeO42− (2 mM) or SeO32− (2 mM) for 0, 10, 20, and 30 min. The Northern blots were hybridized with sodA, trxB, or gor radiolabeled DNA probes. 16S and 23S rRNA were used for loading calibration.