TABLE 3.
Total SOD and catalase activities in E. coli MC4100 crude extracts after selenium oxide treatment
| Enzyme assayeda | Sp act (U/mg) ± SD of:
|
|||||
|---|---|---|---|---|---|---|
| Control at 0 min | SeO42− at:
|
SeO32− at:
|
||||
| 30 min | 120 min | 30 min | 120 min | |||
| Total SOD | 14.7 ± 1.5 | 22.2 ± 2 | 32.3 ± 3 | 35.6 ± 4 | 62.3 ± 6 | |
| Total catalase | 10 ± 1.5 | 17.3 ± 2.6 | 19.2 ± 3 | 38.5 ± 6 | 44.8 ± 7 | |
a
Cells (crude extracts) were treated with 2 mM SeO42− or SeO32− for 30 or 120 min. All assays were performed on exponential-phase cells and monitored by the spectrophotometry direct method as described in Materials and Methods.