TABLE 4.
MnSOD and FeSOD activities in extracts of wild-type and mutant strains
| Strain | SOD | Mean SOD activity ± SDa of:
|
||
|---|---|---|---|---|
| Control | SeO42− | SeO32− | ||
| GC4468 (reference) | MnSOD | 4.8 ± 0.5 | 7.2 ± 0.7 | 13.9 ± 1.5 |
| FeSOD | 5.5 ± 0.6 | 9.6 ± 1 | 12.7 ± 1.5 | |
| QC772 (ΔsodA) | FeSOD | 6.9 ± 0.7 | 11.4 ± 1.5 | 12.1 ± 1.5 |
| QC773 (ΔsodB) | MnSOD | 5.8 ± 0.6 | 8.1 ± 0.8 | 12.6 ± 1.5 |
a
Cells were treated with 2 mM SeO42− or SeO32− for 30 min. The SOD activities (MnSOD, SodA; FeSOD, SodB) are expressed in arbitrary units and are estimated from the gel densitometer tracings of nondenaturing gels.