FIG. 3.
(A) Lane 1, primer extension of mRNA from E. faecalis JH2-2 harboring plasmid pIP501. The experiment was performed with total RNA and primer traP, which overlaps the putative start codon of traA. Lanes A, C, G, and T, corresponding DNA sequence reactions to size the extension products which are marked by arrows. The corresponding pIP501 sequence (complement of the sequence depicted in panel B) is shown on the right. (B) oriT region of pIP501 (nucleotides 1238 to 1417). The transcriptional start site of tra mRNA is marked with a bent arrow and numbered +1. The start of the very weak second extension product (T at position 1304) is underlined. The oriT nick site is indicated by a vertical arrow. The putative −10 and −35 promoter elements are boxed. Arrows below the sequence mark an imperfect inverted repeat with potential to generate a secondary structure. The two possible start codons of traA are indicated, and the putative ribosome binding site is shown in boldface.