TABLE 2.
Enzyme activities of cell extracts of DLHB-grown culturesa
| Strain | Relevant characteristic(s)b | Activity (nmol/min/mg of protein)c of:
|
||
|---|---|---|---|---|
| 3-Hydroxybutyrate dehydrogenase | AACS | LHBCS | ||
| Rm1021 | wt | 44.4 ± 0.8 | 9.8 ± 3.6 | 0 |
| Rm11172 | age-1::Tn5-Tp | 52.7 ± 2.0 | 27.5 ± 2.2 | 40.7 ± 8.5 |
| Rm11175 | bdhA2::Tn5 age-1::Tn5-Tp | 0 | 42.7 ± 7.3 | 83.3 ± 19.3 |
| Rm11192 | bdhA2::Tn5 shb-1 | 0 | 206.8 ± 23.7 | 98.9 ± 5.2 |
| Rm1021(pSP329) | wt (vector) | 52.0 ± 0.9 | 5.9 ± 1.7 | 0 |
| Rm1021(pGQ105) | wt (acsA2) | 42.1 ± 6.8 | 73.9 ± 6.5 | 51.6 ± 19.4 |
| Rm11107(pGQ105) | bdhA1::Tn5 (acsA2) | 0 | 115.2 ± 27.5 | 65.2 ± 12.7 |
a
Cell extracts were prepared from cultures grown in minimal medium supplemented with 15 mM DLHB. Note that bdhA mutant controls could not be included because such strains are unable to grow in DLHB.
b
wt, wild type.
c
Values are averages from triplicate assays ± standard deviations.