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The fibA mutant lacks all MAb 2105 reactive polypeptides. A Western blot with MAb 2105 as primary antibody is shown. Lane 1, lysate from 5 × 108 whole cells of wild-type DK1622; lane 2, 5 μg of protein from purified DK1622 fibrils; lane 3, lysate from 5 × 108 whole cells of LS2200 (fibA); lane 4, 5 μg of protein from purified LS2200 fibrils.
