Skip to main content
. 2002 Mar;184(6):1779–1782. doi: 10.1128/JB.184.6.1779-1782.2002

FIG. 4.

FIG. 4.

Effect of peptide insertions in XcpZ on the functionality of the Xcp machinery of P. aeruginosa. (A) The variant xcpZ genes were subcloned in pMMB67HE and expressed in strain KS902. Elastase LasB was analyzed by immunoblotting in cellular (c) and extracellular (e) fractions obtained from cells grown in the absence of IPTG. (B) Effect of 175 μM IPTG on the functionality of variant XcpZ65, -127, -128, and -141. LasB from cellular and extracellular extracts was analyzed by immunoblotting. At least two independent experiments were carried out, and the results were found to be reproducible. Other legend details are as indicated for Fig. 2.