FIG. 2.

OMP analyses. OMPs were isolated from the wild type, the toxS mutant, the toxR mutant, and the toxR mutant carrying a plasmid-encoded wild-type toxR gene (pToxR) as described in Materials and Methods. These proteins were applied to an SDS-12.5% PAGE gel and separated. The OMPs were stained with Coomassie blue (A) or transferred to nitrocellulose followed by Western blotting using an antiserum raised against OmpU from V. cholerae (B). Lanes 1, 2, 3, and 4 are labeled and are the same for both A and B. Molecular size markers are included in the leftmost lane, and sizes are indicated.