TABLE 1.
B. bronchiseptica strains and alcR+ plasmids used in this study
| Strain or plasmid | Relevant genotype, phenotype, or description | Reference or source |
|---|---|---|
| BRM13 | B. bronchiseptica B013N ΔalcR1 alcA::mini-Tn5 lacZ1; kanamycinr AlcR− alcaligin deficient | 12 |
| BRM13Ω(ΔalcR1::pSS/Bp) | pSS/Bp plasmid integrant derivative of BRM13; AlcR+ kanamycinr ampicillinr gentamicinr | This study |
| BRM13Ω(ΔalcR1::pSS/Bb) | pSS/Bb plasmid integrant derivative of BRM13; AlcR+ kanamycinr ampicillinr gentamicinr | This study |
| BRM13Ω(ΔalcR1::pSS/Bb-S103T) | pSS/Bp-S103T plasmid integrant derivative of BRM13; AlcR(Con) kanamycinr ampicillinr gentamicinr | This study |
| pRK415 | Mobilizable broad-host-range plasmid cloning vector; tetracycliner; RK2 origin | 24 |
| pRK21 | pRK415 with 1.6-kb KpnI-PstI B. pertussis UT25 alcR+ DNA insert fragment; AlcR+ tetracycliner; formerly designated pP9KP | 5, 12 |
| pRK15 | pRK415 with 2.3-kb EcoRI-PstI B. bronchiseptica B013N alcR+ DNA insert fragment; AlcR+ tetracycliner | 12 |
| pRK/1.6KP (Bb) | pRK415 with 1.6-kb KpnI-PstI B. bronchiseptica B013N alcR+ DNA insert fragment; AlcR+ tetracycliner | This study |
| pRK/2.3EP (Bp) | pRK415 with 2.3-kb EcoRI-PstI B. pertussis UT25 alcR+ DNA insert fragment; AlcR+ tetracycliner | This study |
| pBBR1MCS | Mobilizable broad-host-range plasmid cloning vector; chloramphenicolr | 25 |
| pBB/1.6KP (Bp) | pBBR1MCS with 1.6-kb KpnI-PstI B. pertussis UT25 alcR+ DNA insert fragment; AlcR+ chloramphenicolr | This study |
| pBB/1.6KP (Bb) | pBBR1MCS with 1.6-kb KpnI-PstI B. bronchiseptica B013N alcR+ DNA insert fragment; AlcR+ chloramphenicolr | This study |
| pBB/2.3EP (Bp) | pBBR1MCS with 2.3-kb EcoRI-PstI B. pertussis UT25 alcR+ DNA insert fragment; AlcR+ chloramphenicolr | This study |
| pBB/2.3EP (Bb) | pBBR1MCS with 2.3-kb EcoRI-PstI B. bronchiseptica B013N alcR+ DNA insert fragment; AlcR+ chloramphenicolr | This study |
| pRK/NpCb | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment, N-terminal coding sequences of B. pertussis UT25 alcR spliced with C-terminal coding sequences of B. bronchiseptica B013N alcR by overlap extension PCR; AlcR+ tetracycliner | This study |
| pRK/NbCp | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment, N-terminal coding sequences of B. bronchiseptica B013N alcR spliced with C-terminal coding sequences of B. pertussis UT25 lcR by overlap extension PCR; AlcR+ tetracyclineR | This study |
| pRK/NpCp | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment, N-terminal coding sequences of B. pertussis UT25 alcR spliced with C-terminal coding sequences of B. pertussis UT25 alcR by overlap extension PCR; AlcR+ tetracycliner | This study |
| pRK/NbCb | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment, N-terminal coding sequences of B. bronchiseptica B013N alcR spliced with C-terminal coding sequences of B. bronchiseptica B013N alcR by overlap extension PCR; AlcR+ tetracycliner | This study |
| pRK/Bp-G103S | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment encoding B. pertussis UT25 alcR G103S mutant allele, generated by PCR mutagenesis; AlcR+ tetracycliner | This study |
| pRK/Bb-S103G | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment encoding B. bronchiseptica B013N alcR S103G mutant allele, generated by PCR mutagenesis; AlcR+ tetracycliner | This study |
| pRK/Bb-S103T | pRK415 with 1.2-kb KpnI-PstI DNA insert fragment encoding B. bronchiseptica B013N alcR S103T mutant allele, generated by PCR mutagenesis; AlcR(Con) tetracycliner | This study |
| pSS1129 | Mobilizable suicide plasmid; ampicillinr gentamicinr; ColE1 origin | 37 |
| pSS/Bp | pSS1129 with 1.2-kb EcoRI-HindIII DNA insert fragment of pRK/NpCp; AlcR+ ampicillinr gentamicinr | This study |
| pSS/Bb | pSS1129 with 1.2-kb EcoRI-HindIII DNA insert fragment of pRK/NbCb; AlcR+ ampicillinr gentamicinr | This study |
| pSS/Bb-S103T | pSS1129 with 1.2-kb EcoRI-HindIII DNA insert fragment of pRK/Bb-S103T; AlcR(Con) ampicillinr gentamicinr | This study |