TABLE 3.
Genetic complementation of raiI and raiR mutantsa
| Plasmid | AHL productionb detected by CV026 in
|
|||
|---|---|---|---|---|
| 8401 | A789 raiI7::Tn5 | A802 raiR8::Tn5 | A700 | |
| None | + | − | − | − |
| pIJ9228 (raiI raiR) | +++ | +++ | +++ | +++ |
| pIJ9231 (raiI7::Tn5 raiR) | + | − | + | − |
| pIJ9234 (raiI raiR8::Tn5) | + | +/− | − | +/− |
a
AHL production was tested on a plate assay using C. violaceum CV026 as the indicator strain.
b
−, no complementation; +/−, weak complementation (less than 10% of AHL levels produced by 8401); +, complementation; +++, strong complementation (the AHL production level was greater than that of the control 8401).