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. 2002 Mar;184(6):1514–1521. doi: 10.1128/JB.184.6.1514-1521.2002

FIG. 3.

FIG. 3.

Flagellin content in whole-cell lysates of wild-type PAK and flgM mutant PAK-M, analyzed by Western blot with polyclonal anti-FliC rabbit serum. Lanes: 1, wild-type PAK; 2, PAK-M (flgM mutant); 3, pET15bVP vector control in PAK-M; 4, pET15bVP-M plasmid in PAK-M; 5, pMMB67HE vector control in PAK-M; 6, pMMB67HE-M plasmid in PAK-M; and 7, orfA mutant that makes nonglycosylated flagellin. The arrow on the left indicates modified flagellin (45 kDa). The arrow on the right indicates nonglycosylated flagellin (∼40 kDa).