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. 2002 Mar;184(6):1514–1521. doi: 10.1128/JB.184.6.1514-1521.2002

FIG. 4.

FIG. 4.

FlgM-FliA in vivo fusion protein interaction in yeast AH109 transformants. Positive interaction is based on expression of β-galactosidase activity after transcription of the reporter gene lacZ. Panels 1 and 2 show positive β-galactosidase activity in yeast strain AH109 carrying plasmids pGADT7-M and pGBKT7-A and plasmids pGADT7-A and pGBKT7-M, indicating positive interaction between the FlgM and FliA proteins. Panels 3 and 4 show pGADT7-M or pGADT7-A carrying the fliA or flgM, respectively, with the vector control pGBKT7 where no β-galactosidase activity was detected.