FIG. 5.

Growth on n-octane and alkane hydroxylase activity of E. coli GEc137 recombinants containing pGEc47, pGEc47ΔG, or pGEc47ΔG combined with wild-type and mutagenized AlkG2 (A) and AlkG1 (B) Rd domains. Growth on n-octane was documented after 2 and 5 days. Alkane hydroxylase activities are shown to the left of the growth zones. The mutations are shown to the right of the growth zones. For AlkG1, 1 to 7 correspond to plasmids pKK-G1mut1 to pKK-G1mut7. For AlkG2, 1 to 4 correspond to plasmids pKK-G2mut1 to pKK-G2mut4 in Table 1.