TABLE 1.
Plasmids used or constructed in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pAF1 | 3.5-kb EcoRI-BamHI alkGH fragment of A. borkumensis AP1 in pZEr0-2.1 | This study |
| pBG219 | pKK233-2, alkG from pBG214 | 40 |
| pCR14 | pKK233-2, alkG1 from pBG219 by PCR | This study |
| pCR15 | pCR14, AlkG1 N10G A43G | This study |
| pGEc47 | Tc alkBFGHJKL alkST (P. putida GPo1) in pLAFR1 | 7 |
| pGEc47ΔG | Tc alkFGHJKL alkST (P. putida GPo1) in pLAFR1, deletion in alkG | 40 |
| pGEc283 | alkBFGH in pMMB24, 125-bp deletion in alkG | 14 |
| pGEM7-Zf(+) | Cloning vector, Ap | Promega |
| pKK233-2 | Expression vector, Ptrc Ap | 2 |
| pKKPalk | Expression vector, Palk Ap | 35 |
| pKKrubA(ADP1) | pKKPalk, rubA (Acinetobacter sp. ADP1) | This study |
| pKKrubA1(PAO1) | pKKPalk, rubA1 (P. aeruginosa PAO1) | This study |
| pKKrubA2(PAO1) | pKKPalk, rubA2 (P. aeruginosa PAO1) | This study |
| pKKalkG(AP1) | pKKPalk, alkG (A. borkumensis AP1) | This study |
| pKKrubA(H37Rv) | pKKPalk, rubA (M. tuberculosis H37Rv) | This study |
| pKKrubB(H37Rv) | pKKPalk, rubB (M. tuberculosis H37Rv) | This study |
| pKKrubA1(Rer) | pKKPalk, rubA1 (R. erythropolis NRRL B-16531) | This study |
| pKKrubA2(Rer) | pKKPalk, rubA2 (R. erythropolis NRRL B-16531) | This study |
| pKKrubA3(Rer) | pKKPalk, rubA3 (R. erythropolis NRRL B-16531) | This study |
| pKKrubA4(Rer) | pKKPalk, rubA4 (R. erythropolis NRRL B-16531) | This study |
| pKKrubG4W(Aru) | pKKPalk, rub domain (P. rugosa NRRL B-2295) | This study |
| pKKrubG4W(Aru) | pKKPalk, rub domain (P. rugosa NRRL B-2295) | This study |
| pKK-G1 | pKKPalk, alkG1 domain (P. putida GPo1) | This study |
| pKK-G1mut1 | pKK-G1, N10 changed to G | This study |
| pKK-G1mut2 | pKK-G1, H21 changed to A | This study |
| pKK-G1mut3 | pKK-G1, PWHL29-32 changed to RWED | This study |
| pKK-G1mut4 | pKK-G1, AVRD43-46 changed to GAT | This study |
| pKK-G1mut5 | pKK-G1, R45 changed to A | This study |
| pKK-G1mut6 | pKK-G1, N10 changed to G and AVRD43-46 changed to GAT | This study |
| pKK-G1mut7 | pKK-G1, N10 changed to G, PWHL29-32 changed to RWED, and AVRD43-46 changed to GAT | This study |
| pKK-G2 | pKKPalk, alkG2 domain (P. putida GPo1) | This study |
| pKK-G2mut1 | pKK-G2, G10 changed to N | This study |
| pKK-G2mut2 | pKK-G2, A21 changed to H | This study |
| pKK-G2mut3 | pKK-G2, RFED29-32 changed to PFHL | This study |
| pKK-G2mut4 | pKK-G2, GAT43-46 changed to AVRD | This study |
| pP1EH | pBR322, alkBFGHJ (P. putida P1) | 34 |
| pZErO-2.1 | Cloning vector, Km ccdB | Invitrogen |