TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Bacterial strains | ||
| E. faecalis | ||
| KBL 703 | Original lysogenic strain for phage ΦFC1 | 8, 35 |
| KBL 707 | Indicator strain for phage ΦFC1 | 29 |
| E. coli JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 (lac-proAB) F traD36 proAB lacIqZM15 | 38 |
| Plasmids | ||
| pESH2.8 | Ampr Cmr; replicative ori of ColE1; pEK104 carrying a 2.8-kbp HindIII fragment of pS3.6 | 25 |
| pCONT3.7 | Ampr Cmr; replicative ori of ColE1 | This study |
| pEMJ1-1 | pCONT3.7 carrying a 2,744-bp attP fragment and int fragment of phage ΦFC1 | This study |
| pATTB1 | pACYC carrying 290-bp attB fragment | This study |
| pETMJ1 | pET14b carrying int and attP | 23 |
| pREC1 | Plasmid by recombination of pETMJ1 and pATTB1 | This study |
| pT7BlueR | Ampr; T cloning vector | Novagen |
| pATTR | pT7BlueR carrying the attR region | This study |
| pATTL | pT7BlueR carrying the attL region | This study |