TABLE 1.
E. coli K-12 strains
| Strain | Genotypea | Source, reference, or construction |
|---|---|---|
| LJ24 | thi-1 leu-6 lacY1 lacI-ZΔ(Mlu) glnV44 tonA21 rpsL rfbD1 rpoS396(Am) | 6, 36 |
| BBC119 | λRB1b | 17 |
| MC4100 | araD139 Δ(argF-lac)U169 deoC1 flb5301 relA1 rpsL150 ptsF25 rbsR | 38 |
| GM230 | hns-205::Tn10 φ(proU-lacZ)hyb2(λ placMu15)c | 26 |
| PD23 | hns-206::Aprc | 19 |
| RH90 | rpoS359::Tn10c | 30 |
| BL21/λDE3 | gal met r− m−hsdS placIq/λ placUV5-T7-gene 1 | 41 |
| TC4061 | λRB1 hns-205::Tn10b | GM230(P1) × BBC119 |
| TC4136 | λRB1 hns206::Aprb | PD32(P1) × BBC119 |
| TC4409 | λRB1 rpoS359::Tn10b | RH90(P1) × BBC119 |
| TC4410 | λRB1 rpoS359::Tn10 hns206::Aprb | RH90(P1) × TC4136 |
| TC3983 | attB::p appY′-lacZb | 15 |
| TC4033 | hns205::Tn10 attB::pappY′-lacZb | 7 |
| TC4135 | hns206::AprattB::pappY′-lacZb | 7 |
| TC4300 | attB::pmioC4140-lacZb | This work |
| TC4301 | attB::pmioC4141-lacZb | This work |
| TC4302 | attB::pmioC4142-lacZb | This work |
| TC4623 | hns206::AprattB::pmioC4140-lacZb | PD32(P1) × TC4300 |
| TC4624 | hns206::AprattB::pmioC4141-lacZb | PD32(P1) × TC4301 |
| TC4625 | hns206::AprattB::pmioC4142-lacZb | PD32(P1) × TC4302 |
| TC4617 | hns206::AprattB::pappY′-lacZb/pTAC4617 | This work |
a
Genetic symbols are as described elsewhere (9).
b
Genotype otherwise like LJ24.
c
Genotype otherwise like MC4100.