TABLE 2.

Comparison of cell cycle and initiation parameters in the wild type and the hns206 mutant^a

Growth temp (°C)	Genotype	Origins/cell	DNA (g.e.)b/cell	tD (min)	C + Dc (min)	Ce (min)	Df (min)	Cell size (LS/cell)g	Origins/LSh	DnaA-β-galactosidase contenti
37	hns+	7.2	5.0	27	75	41	40	1.00	1.00	1.00
	hns206	4.1	3.4	31	63	34	36	0.69	0.84	1.28
30	hns+	7.1	5.0	44	124	62	69	0.97	1.01	1.00
	hns206	3.1	2.5	58	95	29	62	0.64	0.67	1.42
14	hns+	6.2	3.1	450	NCd	630	400	0.86	1.00	NDj
	hns206	3.5	2.4	960	NC	750	800	0.91	0.54	ND

^aStrains BBC119 and TC4136 were grown in glucose-Casamino Acids-supplemented medium at the indicated temperatures. Samples were obtained for flow cytometric analysis of DNA, origins, and light scatter (LS); for Southern blot analysis of origins per termini (O/T); and for determination of DnaA-β-galactosidase specific activity.

^bg.e., genome equivalents.

^cDetermined as: origins per cell = 2^(C+D)/t_D.

^dNC, not calculated due to rifampin-resistant initiations (3).

^eDetermined as: O/T = 2^C/t_D.

^fDetermined as: g.e./cell = [(O/T − 1)/ln(O/T)]·2^D/t_D (24).

^gNormalized to the value for BBC119 grown at 37°C.

^hThat is, (origins/cell)/(LS/cell) normalized to the value for BBC119 grown at 37°C.

ⁱMeasured as the specific activity normalized to that of BBC119 grown at the same temperature.

^jND, not determined.