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Disruption of C. albicans CBK1. (A) Partial restriction map of the CBK1 locus. The HindIII/EcoRI fragment was subcloned into pUC18, generating pMM1. The BglII/XbaI fragment of the CBK1 locus was replaced with the BglII/PstI hisG-URA3-hisG fragment to construct the disruption cassette. (B) Southern blot of genomic DNA digested with HpaI and hybridized with a CBK1-specific probe. Genotypes of the strains are indicated above each lane.
