Acinetobacter strains |
|
|
ADP1 |
Wild type (strain BD413, ATCC 33305) |
26 |
ADPU6 |
PcaU binding site downstream of pcaU replaced by Kmr cassette |
This study |
E. coli strains |
|
|
DH5α |
General-purpose cloning strain |
20 |
BL21(DE3) |
F−ompT hsdSB(rB−mB−) gal dcm (DE3); gene for T7 RNA polymerase under control of lacUV5 integrated into the chromosome |
39 |
Plasmids |
|
|
pBend5 |
Vector designed for circular permutation assay based on pBluescript SK(−) |
46 |
pET-21(+) |
Transcription vector with T7 promoter |
39 |
pKOK6 |
Contains promoterless lacZ-Kmr cassette |
28 |
pZR15 |
7-kbp HpaII fragment containing pcaU inserted into pUC19 |
17 |
pZR17 |
2,792-bp EcoRI fragment from pZR15 cloned into pUC19 |
17 |
pAC7 |
2,792-bp EcoRI fragment from pZR15 cloned into pET-21(+), T7 promoter upstream of pcaU
|
This study |
pZR18 |
2,618-bp HindIII-HpaII fragment containing pcaU inserted into pUC19 |
17 |
pAC25 |
Corresponds to pZR18 after removal of 218-bp SwaI-Eco47III fragment |
This study |
p5/128 |
128-bp EcoRI-BstUI fragment of the pcaU-pcaI intergenic region in HpaI site of pBend5 |
This study |
pAC29 |
pZR17 with 130-bp BstBI fragment replaced by Kmr cassette from pKOK6 |
This study |
pAC48 |
Contains pcaI′-pcaU, lacZ-Kmr cassette from pKOK6 fused to pcaI′ on vector pRK415 |
P. Patz and U. Gerischer, unpublished results |
pAC52 |
130-bp BstBI fragment deleted from pAC48 |
This study |
p5DR |
Intergenic PcaU binding site without the external inverted 10-bp sequence repetition on vector pBend5 |
This study |