Restriction map of pAP45 and domain structure of AprIV. (A) Restriction map of pAP45. Arrow indicates the ORF and the direction of transcription. aprIV, the gene encoding serine protease. (B) Diagram of domain structure of AprIV: signal peptide, ▪; N-terminal proregion, ▤; protease region, □; PkdD, ▩; ChtBD, ▨.