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Effects of pH and temperature on protease activity of AprIV. (A) The following buffer systems were used: 50 mM acetate buffer (pH 6.0), 50 mM Tris-HCl buffer (pH 7.0 to 9.0), and 50 mM glycine-NaOH buffer (pH 10.0 to 13.0). The reaction mixtures were incubated at 35°C for 30 min. The amount enzyme was 13.6 U. (B) The reaction was carried out at the various temperatures for 30 min at pH 11.5 (50 mM glycine-NaOH buffer). The amount of enzyme was 13.6 U.
