FIG. 7.

 Particitation of AprIV and AprIVΔC in chitin degradation. (A) Protease activities of AprIV and AprIVΔC were measured with the substrates native chitin (open bar) and deproteinized chitin (solid bar). Control experiments were carried out without enzyme. (B and C) The effects of AprIV and AprIVΔC on chitinase activity were examined by using native chitin (B) or deproteinized chitin (C). Symbols: •, Chi85; ▴, Chi85 plus AprIVΔC; ▪, Chi85 plus AprIV. Aliquots were taken at the indicated times and centrifuged, and chitinase activity was measured. The value after incubation for 6 h with AprIV and Chi85 was taken as 100%. Data from three independent experiments are shown; standard deviations are indicated by vertical lines.