FIG. 3.

Colorimetric detection of His-tagged AdhE and butanol dehydrogenase domain in recombinant C. acetobutylicum strains. Cell extracts were separated by SDS-polyacrylamide gel electrophoresis and were transferred onto a nitrocellulose membrane. Subsequently, His-tagged proteins were detected with Ni-AP conjugate. The molecular sizes of marker proteins are indicated on the left. Lane S, marker proteins; lane 1, C. acetobutylicum(pKLIMP7): lane 2, C. acetobutylicum(pKLIMP12); lane 3, C. acetobutylicum(pKLIMP17).