TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description or genotype | Source or reference |
|---|---|---|
| Escherichia coli | ||
| ER2275 | trp-31 his-1 tonA2 rpsL104 supE44 xyl-7 mtl-2 metB1 e14− Δ(lac)U169 endA1 recA1 R(zbgZ10::Tn10) Tcs Δ(mcr-hsd-mrr)114::1510 F′ [proAB traD36 laq1qΔM15 zzf::mini Tn10 (Kmr)] | 20 |
| WL3 | adhC81 fadR adhE3 | 17 |
| XL1-B | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 [F′ proAB lac1qZΔM15 Tn10 (Tcr)] | Stratagene GmbH, Heidelberg, Germany |
| Clostridium acetobutylicum DSM 792 | Wild type | DSMZ, Braunschweig, Germanya |
| Plasmids | ||
| pACT1 | C terminus orf5 and intergenic region between orf5 and P1sol in pIMP1 | This study |
| pAN1 | Cmr, φ3tI, p15A oriR | 20 |
| pGP1-2 | Tn903 (Kmr), cI857, T7 polymerase under control of λPL, P15A oriR | 32 |
| pIMP1 | Emr (ermC) Apr, pMB1 oriR, pIM13 oriR(+) | 19 |
| pK9 | Apr, ColE1 oriR, rop, harboring sol and orf5 | 9 |
| pKLIMP7 | P1-His6-tagged adhE fusion in pIMP1 | 23 |
| pKLIMP12 | P2-[ΔP1]-His6-tag-encoding adhE fusion in pIMP1 | 23 |
| pKLIMP17 | P1-[Δ hairpins upstream of the adhE start codon]-His6-tag-encoding adhE fusion in pIMP1 | 23 |
| pLacZF | lacZ from T. thermosulfurigenes in pIMP1 | This study |
| pSN51 | Apr, tetM, pMB1 oriR, pAMβ1 oriR(+), oriT, Padc-lacZ-fusion | 23 |
| pTWa4-2 | Apr, ColE1 oriR, f1 ori, T7 promoter, atpE TIR | 36 |
| pTWa4-2::adhE6×His | His6-tag-encoding adhE in pTWa4-2 | This study |
| pZF-sol | P1-lacZ fusion in pLacZF | This study |
| pZF-sol-act | P1-[Δ activator region]-lacZ fusion in pLacZF | This study |
| pZF-sol-0A | P1-[mutated Spo0A-binding site]-lacZ fusion in pLacZF | This study |
| pZF-sol-R1 | P1-[mutated activator region]-lacZ fusion in pLacZF | This study |
| pZF-sol-R2 | P1 [mutated activator region]-lacZ fusion in pLacZF | This study |
| pZF-sol-R3 | P1-[mutated activator region]-lacZ fusion in pLacZF | This study |
| pZF-sol-R1/R2 | P1-[mutated activator region]-lacZ fusion in pLacZF | This study |
a
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen.