TABLE 2.
Deoxyoligonucleotides used in this study
| Primer | Sequence (5′ → 3′)a |
|---|---|
| KLADHE01 | TATTTTAGAAAGAAcTGcAgAcaTATGAAAGTCAC |
| KLADHE02 | CCTCCTTTTAAATTCTTTATgAAttcTtagtgAtGgtGaTGgtgatgaggcGTTggAGGTTGTTTTTTAAAAC |
| KL06 | GGGAATACCATAtgTcgACACTTCTTTCTAAAATA |
| KL07 | CGgAaTtCATTTAAATACACAGCTG |
| KL11 | AGATCgataTcggtAaccTCATAAAATTTATGATCC |
| KL12 | AGATCgatatcTTAAATACACAGCTG |
| KL17 | AAAAACAATATtGACATaATaTTcAAgATATAATAAAT |
| KL18 | ATTTATTATATcTTgAAtATtATGTCaATATTGTTTTT |
| AdhE-PE-nat-IRD | CTTCTTTAATTACCTTGAGT |
| AdhE-PE-klimp-IRD | GTGACTTTCATATGTCGACAC |
| 5-inter-sol-eco | GTAATTgAATTcAATGATTTAGGCATAGAAATCG |
| 5-inter-sol-pst | AATActgCAGCTGTGTATTTAAATGTAAATAGC |
| lacZ-5-Sal-frame | GGAAGGTGACTgTcgacAGAAAGATTATTCC |
| lacZ-3-Pst-end | GCTTATATAATCTGcAGATGAAATTCTC |
| adhE-fus-up-Sal | AATTCgTcgACTGTTGTGACTTTCATAAATATACAC |
| PadhE+Act-Bam | TATTGGatCcATTAATTAGGGTTATATATACTAG |
| PadhE-Act-Bam | AAAAGgatCcATTTACATTTAAATACACAGCTG |
| spo0A-up | GGAATCTAATATTTTGGaactATAATATATATTTAGG |
| spo0A-down | CCTAAATATATATTATagttCCAAAATATTAGATTCC |
| R1-up | GGAATTATCAGTAaAcTaAtAAAAATATGAAGG |
| R1-down | CCTTCATATTTTTaTtAgTtTACTGATAATTCCTAA |
| R2-up | GCGTAATAATATAaAcTaAtGAATTATCAGTATATTTAG |
| R2-down | CTAAATATACTGATAATTCaTtAgTtTATATTATTACGCC |
| R3-up | CAAAATGGTATCTAAcAagaTGGCGTAATATATATTTAGG |
| R3-down | ATATATATTACGCCAtctTgTTAGATACCATTTTGAAAAG |
| R1/2-up | CGTAATATAaAcTaAtGAATTATCAGTAaAcTaAtAAAAATATG |
| R1/2-down | CATATTTTTaTtAgTtTACGTATAATTCaTtAgTtTATATTACG |
a
Substituted bases are indicated by lowercase letters; introduced restriction sites are indicated by italics.