TABLE 2.
Oligonucleotide primers used
| Primer | Sequence (5′-3′) | Location or description |
|---|---|---|
| OS1 | AAGCAACTTCTCATATTGATACGGAGACGG | 5′ region of truncated orf100 |
| OS2 | CCACGCTTGATCCAATACAGGAAATTGTGC | 5′ region of truncated ptsG |
| SL1 | TACATTGATAATCCGCCAGC | 5′ region of sly |
| SL4 | AAACTGTTCTCCACCATTCC | 3′ region of sly |
| ORF102-1 | ACGAGAAAACCTTGCGACTG | 5′ region of orf102 |
| ORF102-2 | CTGGATTGATAGGAGTGTTG | 3′ region of orf102 |
| ORF102-3 | GTCAAGAAAAATAATGGCGG | Just downstream of ORF102-1 |
| ORF100-1 | CTATCTCTTTACAGGGACGA | 5′ region of truncated orf100 |
| ORF100-2 | ACACCTTTGCTTGAATCTCA | 3′ region of truncated orf100 |
| SD1 | AGGTGAATTCGTTTGAACGTGCTTTGG | 5′ region of orf101 |
| ORF101-2 | AACGTTCTTCCATTAGTTGA | 3′ region of orf101 |
| nanE1 | TTTCCTGTCAGGCTTTGCCA | 5′ region of nanE |
| nanE2 | TTCCTTTGGACGTGTGATCG | 3′ region of nanE |
| ptsG1 | TGTTGCTGGTCTCTTACTGG | 5′ region of truncated ptsG |
| ptsG2 | CCATACCAGGAATTAGCACGTGAAAT | 3′ region of truncated ptsG |
| SD2 | CGCAGGATCCAATACAGGAAATTGT | 5′ region of truncated ptsG |
| F1 | GAGTTTGATCCTGGCTCAG | 5′ region of 16S rRNA |
| R13 | AGAAAGGAGGTGATCCAGCC | 3′ region of 16S rRNA |