FIG. 2.

PH activity response to purine present in culture medium. (A) PH specific activities from cell extracts of C. purinolyticum cultured on different purines (12 mM) except where indicated. Activities given represent the mean derived from at least three independent cultures, and error bars indicate 1 SD from the mean. A*, adenine-grown cell extract (1 mM); A, adenine-grown cell extract; P, purine-grown cell extract; HX, hypoxanthine-grown cell extract; X, xanthine-grown cell extract; U, uric acid-grown cell extract. (B) Western analysis of the small subunit of PH from an extract of cells grown on each purine at a 12 mM concentration in the medium. A, adenine-grown cell extract (10 μg); P, purine-grown cell extract (10 μg); HX, hypoxanthine-grown cell extract (10 μg); X, xanthine-grown cell extract (10 μg); U, uric acid-grown cell extract (10 μg).