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. 2002 Apr;184(7):2039–2044. doi: 10.1128/JB.184.7.2039-2044.2002

FIG. 5.

FIG. 5.

Responses of PH and XDH activities to the availability of molybdenum in the culture medium. (A) XDH specific activities in extracts of C. purinolyticum cells cultured on various concentrations of molybdate as indicated. (B) PH specific activities in extracts of C. purinolyticum cells cultured on various concentrations of molybdate as indicated. Activities given represent the mean derived from at least three independent cultures, and error bars indicate 1 SD from the mean. Cells were cultured with uric acid (•) and adenine (▴). (C) Western analysis of small subunit of PH from a representative extract of cells grown in various concentrations of molybdate. The concentration of molybdate in the growth medium is indicated below each lane, and 10 μg of cell extract was added to each lane. The purines added to the growth medium are also indicated.