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Western blot analysis of NifH modification after ammonium addition to nitrogen-fixing cultures of Azoarcus sp. wild-type strain BH72 (A) and BHGLBS (glnB) (B), BHGLKK (glnK) (C), BHGLKKBS (glnBK) (D), and BHABK (amtB) (E). Samples were taken before (lane 1) or 5 min (lane 2) or 15 min (lane 3) after addition of 2 mM NH4Cl. Experiments were performed at least two times.
