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Western blot analysis of NifH modification upon anaerobiosis. N2-fixing cultures of the Azoarcus sp. were transferred from microaerobic conditions (0.8% oxygen) (A) to anaerobic conditions. Samples were taken after 5 min of anaerobic incubation (B). Lanes: wild-type strain BH72 (lane 1), glnB (lane 2), glnK (lane 3), amtB (lane 4), and glnBK (lane 5) mutant strains.
