TABLE 1.
Bacterial strains, cloning vectors, and recombinant plasmids used
| Strain, vector, or plasmid | Relevant genotype or properties | Source or reference |
|---|---|---|
| E. coli DH5α | F′ recA1 endA1 hsdR17(rK− mK+) supE44 (λ−thi-1 relA1 φ80d lacZΔM15 Δ(lacZYA-argF′)U169 | 19 |
| Azoarcus sp. | ||
| BH72 | Wild type | 49 |
| BHGLKK | Kmr, BH72 glnK::Kmr | 39 |
| BHGLBS | Sm/Spr, BH72 glnB::Sm/Spr | 39 |
| BHGLKKBS | Kmr, Sm/Spr, BH72 glnK::Kmr, glnB::Sm/Spr | 39 |
| BHABK | Kmr, BH72 amtB::Kmr | This study |
| Cloning vectors | ||
| pBluescript SK | Apr, ColE1 origin | Stratagene |
| pBK-CMV | Kmr, Neor, ColE1 origin, fl(−) origin, simian virus 40 origin | Stratagene |
| pUC4K | Kmr, Neor, ColE1 origin | Pharmacia |
| pLAFR3 | Tetr, low-copy cosmid vector | 56 |
| Recombinant plasmids | ||
| pDZD17 | Kmr, glnk-amtB locus on a 5.8-kb chromosomal SauIIIAI fragment of strain BH72 in pBK-CMV | 39 |
| pDZD17.1 | Apr, 3.6-kb HindIII/SpeI fragment of pDZD17 cloned in pBluescript SK | This study |
| pDM3 | Apr, Kmr, 1.3-kb Kmr cassette of pUC4K cloned in SrfI restriction site of pDZD17.1 (600 bp downstream of amtB start codon) | This study |
| pEGN3.1 | Apr, nifH::uidA transcriptional fusion on pUC19 | 12 |
| pNHGus | Tetr, 3.3-kb HindIII/EcoRI fragment harboring nifH::uidA fusion of pEGN3.1 cloned in pLAFR3 | This study |
| pKKOM | Tetr, 1.5-kb SrfI/KpnI fragment of pDZD17 cloned in pLAFR3 | This study |