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Mutation of the waaF gene in C. jejuni NCTC 11168 affects the size and antigenicity of core oligosaccharide. SDS-PAGE gels of LOS extracts of wild-type C. jejuni NCTC 11168 and waaF mutants were silver stained (A) or immunoblotted with HS:2 antisera (B). The truncated LOS is not detectable by Western blotting using HS:2 antisera. Lane 1, waaF mutant 11168NOL22 (cat forward orientation); lane 2, waaF mutant 11168NOL23 (cat reverse orientation); lane 3, NCTC 11168.
