TABLE 1.
Regulation of lacZ fusions in response to methionine and MTA
| Fusion | Strain | Relevant genotype | β-Galactosidase activitya
|
|||
|---|---|---|---|---|---|---|
| −Met | +Met | +MTA | +Met, +MTA | |||
| ykrT-lacZ | BR151-ZKO | metB tyrZ::neo | 130 | 0.10 | 7.9 | 0.18 |
| ykrT-lacZ | BR151-YkrTKO | metB ΔykrT tyrZ::neo | 70 | 0.14 | 77 | 0.23 |
| ykrT-lacZ | BR151-YkrSKO | metB ykrS::neo | 50 | 0.11 | 61 | 0.11 |
| ykrW-lacZ | BR151-ZKO | metB tyrZ::neo | 38 | 0.63 | 8.0 | 0.92 |
| yitJ-lacZ | BR151 | metB | 340 | 0.17 | 10 | 0.32 |
a
Cultures were grown in Spizizen minimal medium (1) containing methionine (0.34 mM), split, and grown in the presence or absence of methionine and/or MTA (0.4 mM). Samples were taken 4 h after the cultures were split. β-Galactosidase activities are in Miller units (12).