FIG. 1.

Linear map of pAL5000 (4,837 bp), showing the locations of the open reading frames (ORF1 to ORF5), the origin of replication (ori), and the relevant restriction sites. The locations of the low-affinity (L) and high-affinity (H) binding sites of RepB described previously (20) are indicated. The region enclosed in a box (200 bp) was used as a probe in EMSA. The in-frame fusions were fusions either with the six-histidine tag (6X His) or with MBP. In pTAB2 the expressed protein lacks any extra amino acid residues at the N terminus.