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Antibody supershift assays performed with either pAB1 or pTAB2 extracts (A) and with extracts of mycobacterial cells harboring pMC2 [Myc(pMC2)] (B). I, 1 μl of anti-RepB immune serum was added to the assay mixture; P, 1 μl of preimmune serum was added to the assay mixture. F, free probe.
