TABLE 1.
Vectors used in this study
| Plasmid(s) | Description |
|---|---|
| pMC2 | Contains the 2.56-kb EcoRV-HpaI fragment (nucleotides 3895 to 1257) derived from the pAL5000 replication region |
| pQE30, -31, -32 | Expression vectors for E. coli; polypeptides expressed from these vectors contain an affinity tag of six histidine residues fused to the N terminus |
| pMALc2 | Expression vector for E. coli; polypeptides expressed from this vector contain MBP as the affinity tag |
| pT7.7 | T7 promoter-based expression systema |
| pAB1 | repA and repB genes expressed from pQE31; repA is fused in frame with the polyhistidine tag |
| pAB1.1 | Similar to pAB1, but repA is fused out of frame with the polyhistidine tag; does not express repA |
| pAB1.2 | repB region deleted from pAB1 |
| pAB2 | repB gene expressed from pQE30 |
| pAB2a | Same as pAB2, but carrying a p15A origin of replication instead of the usual ColE1 origin; vector pTAB2a is compatible with pAB1.2 in E. coli |
| pTAB2 | RepB expressed as untagged protein from vector pT7.7 |
| pMAB1 | repA and repB genes expressed from pMA1-c2; repA is fused in frame with MBP |
a
See reference 22.