FIG. 2.

SecG dependence of secY mutants. Strains KC9 (secY⁺ ΔsecG::kan), KC10 (secY205 ΔsecG::kan), KC11 (secYΔ5 ΔsecG::kan), KC12 (secYΔ6 ΔsecG::kan), and KC13 (secYΔ7 ΔsecG::kan), all of which carried psecG⁺ (plac-secG), were grown in peptone-IPTG medium at 37°C until mid-log phase. Then 2 μl of a 100-fold diluted culture was spotted onto peptone-IPTG agar (+) or L-agar (−) and incubated at 37°C for 12 h.