TABLE 2.
Plasmids
| Plasmid | Cloned genesa | Replicon | Reference |
|---|---|---|---|
| pCJ1 | rpmD-rplO-secYΔ1-rpmJ | pACYC184 | This study |
| pCJ2 | rpmD-rplO-secYΔ2-rpmJ | pACYC184 | This study |
| pCJ3 | rpmD-rplO-secYΔ3-rpmJ | pACYC184 | This study |
| pCJ4 | rpmD-rplO-secYΔ4-rpmJ | pACYC184 | This study |
| pCJ5 | rpmD-rplO-secYΔ5-rpmJ | pACYC184 | This study |
| pCJ6 | rpmD-rplO-secYΔ6-rpmJ | pACYC184 | This study |
| pCJ7 | rpmD-rplO-secYΔ7-rpmJ | pACYC184 | This study |
| pCJ8 | rpmD-rplO-secYΔ8-rpmJ | pACYC184 | This study |
| pCJ9 | rpmD-rplO-secYΔ9-rpmJ | pACYC184 | This study |
| pCJ10 | rpmD-rplO-secYΔ10-rpmJ | pACYC184 | This study |
| pCJ11 | rpmD-rplO-secYΔ11-rpmJ | pACYC184 | This study |
| pCJ12 | rpmD-rplO-secYΔ12-rpmJ | pACYC184 | This study |
| pCJ13 | rpmD-rplO-secYΔ13-rpmJ | pACYC184 | This study |
| pCJ14 | rpmD-rplO-secYΔ14-rpmJ | pACYC184 | This study |
| pCJ15 | rpmD-rplO-secYΔ15-rpmJ | pACYC184 | This study |
| pCM10 | rpmD-rplO-secY+-rpmJ | pACYC184 | 18 |
| pCM56 | syd | pBR322 | Matsuo et al., unpublished |
| pSTV28 | Vector | pACYC184 | 8 |
| pSTD342 | lacIq | pACYC184 | Akiyama et al., unpublished |
| p secG+ | secG+ | pBR322 | Matsumoto et al., unpublished |
a
Only the intact genes are shown, and those relevant to this study are shown in boldface. All the cloned genes except lacIq were under lac promoter control. The antibiotic resistance marker on pCM56 and psecG+ was ampicillin, and that on the other plasmids was chloramphenicol.