TABLE 3.
Primers used for site-directed mutagenesis
| secY mutation | Sequencea |
|---|---|
| secYΔ1 | 5′ CTGAAAGGCTACGGCTAATAATTGGTCGCCCG3′ |
| secYΔ2 | 5′ CTGAAAGGCTACTGACGATAATTGGTC3′ |
| secYΔ3 | 5′ GAACCTGAAAGGCTAAGGCCGATAATTGGTC3′ |
| secYΔ4 | 5′ GGCGAACCTGAAATGATACGGCCGATAATTG3′ |
| secYΔ5 | 5′ GAAGGCGAACCTGTAAGGCTACGGCCG3′ |
| secYΔ6 | 5′ GAAGAAGGCGAACTAGAAAGGCTACGGCC3′ |
| secYΔ7 | 5′ CATTGAAGAAGGCGTAACTGAAAGGCTACGGC3′ |
| secYΔ8 | 5′ GCATTGAAGAAGTAGAACCTGAAAGGC3′ |
| secYΔ9 | 5′ GTCTGCATTGAAGTAGGCGAACCTGAAAG3′ |
| secYΔ10 | 5′ GAGTCTGCATTGTAGAAGGCGAACCTG3′ |
| secYΔ11 | 5′ GTATGAGTCTGCATAGAAGAAGGCGAACC3′ |
| secYΔ12 | 5′ CAGTATGAGTCTTAATTGAAGAAGGCG3′ |
| secYΔ13 | 5′ CCAGTCAGTATGAGTAAGCATTGAAGAAGGC3′ |
| secYΔ14 | 5′ GTCCAGTCAGTATTAGTCTGCATTGAAGAAG3′ |
| secYΔ15 | 5′ GATGTCCAGTCAGTAAGAGTCTGCATTGAAG3′ |
a
Only one of the complementary sequences is shown for each mutation. Chain-terminating codons are shown in boldface.