TABLE 3.
Intracellular transmission by Tra+ and Tra− plasmidsa
| Expt | Replicate | Transmission frequency
|
|||
|---|---|---|---|---|---|
| RP4 | pRK21558 | F42::mini-Tn10Kn | Jp143 | ||
| 1 | 1 | 2 × 10−4 (142)b | ≤2 × 10−6 (0) | 4 × 10−4 (60) | ≤1 × 10−5 (0) |
| 2 | 3 × 10−4 (126) | ≤1 × 10−6 (0) | 7 × 10−4 (63) | ≤7 × 10−6 (0) | |
| 3 | 4 × 10−4 (128) | ≤2 × 10−6 (0) | 2 × 10−4 (45) | ≤1 × 10−5 (0) | |
| 2 | 1 | 1 × 10−4 (43) | ≤2 × 10−6 (0) | 2 × 10−3 (123) | ≤2 × 10−5 (0) |
| 2 | 1 × 10−4 (84) | ≤1 × 10−6 (0) | 9 × 10−4 (428) | ≤1 × 10−6 (0) | |
| 3 | 1 × 10−4 (48) | ≤1 × 10−6 (0) | 1 × 10−3 (335) | ≤1 × 10−6 (0) | |
| 3 | 1 | 3 × 10−4 (382) | ≤4 × 10−6 (0) | 2 × 10−4 (272) | ≤2 × 10−7 (0) |
| 2 | 4 × 10−4 (417) | ≤2 × 10−5 (0) | 6 × 10−4 (266) | ≤7 × 10−7 (0) | |
| 3 | 3 × 10−4 (523) | ≤7 × 10−6 (0) | 4 × 10−4 (112) | ≤4 × 10−7 (0) | |
| Avg | 2 × 10−4 ± 6 × 10−5 (1,893) | 7 × 10−4 ± 3 × 10−4 (1,704) | |||
a
In IncP experiments, the donor was either SL1344R (RP4) or SL1344R(pRK21558) and the recipient was SL1344N. In IncF experiments, the donor was either 14028R-P(F42::mini-Tn10Kn) or 14028R-P(Jp143) and the recipient was BA770. Plasmid transmission frequencies are expressed as the number of transconjugant colonies per limiting intracellular parent.
b
The numbers in parentheses are the numbers of recombinant colonies observed.