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Uptake of [14C]Glc6P into IPTG-induced E. coli cells (uhpT::Tn1000) harboring plasmid uhpC/pET16b. (A) Time dependency of [14C]Glc6P uptake (at a concentration of 25 μM). (B) Substrate saturation of [14C]Glc6P uptake. Cells were incubated for 1 min with the indicated concentrations. Inset: Hanes plot of the [14C]Glc6P uptake, indicating an apparent Km of 25 μM and a Vmax of 38 nmol per mg of protein per h.
