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. 2002 Apr;184(8):2108–2115. doi: 10.1128/JB.184.8.2108-2115.2002

FIG. 7.

FIG. 7.

Complementation of the uhpC-deficient E. coli strain BL21 (uhpC::Tn1000) with the plasmids uhpC/pET16b or hptcp/pET16b. The corresponding suspensions were induced with IPTG and/or with 100 μM Glc6P. For determination of uptake rates the cells were incubated for 1 min with 10 μM [14C]Glc6P. In column 5, 10, and 12 Fru6P (200 μM) was added during [14C]Glc6P uptake. Because of the experimental design, the background activity (−IPTG, −Glc6P) was not subtracted. Columns: 1, wild-type E. coli BL21, −IPTG, −Glc6P; 2, wild-type E. coli BL21, −IPTG, +Glc6P; 3, uhpC-deficient E. coli BL21 (uhpC::Tn1000), complemented with uhpC/pET16b, −IPTG, −Glc6P; 4, see column 3, −IPTG, +Glc6P; 5, see column 3, −IPTG, +Glc6P, +Fru6P; 6, see column 3, +IPTG, +Glc6P; 7, uhpC-deficient E. coli BL21 (uhpC::Tn1000), complemented with hptcp/pET16b, −IPTG, −Glc6P; 8, see column 7, −IPTG, + Glc6P; 9, see column 7, + IPTG, −Glc6P; 10, see column 7, + IPTG, −Glc6P, +Fru6P; 11, see column 7, +IPTG, +Glc6P; 12, see column 7, +IPTG, +Glc6P, +Fru6P.