FIG. 2.

Primer extension analysis of ansZ. B. subtilis RNA used in the primer extension was isolated from wild-type cells grown in glucose minimal medium containing either glutamate plus ammonium (lane 1) or glutamate (lane 2) as the nitrogen source. S. cerevisiae RNA was used in the primer extension shown in lane 3. The ANSZ7 oligonucleotide primer was used for dideoxynucleotide sequencing of pANS41 (lanes A, C, G, and T) and for primer extensions. The primer extension product is denoted by the arrow. Identical results were obtained with the ANS6 oligonucleotide primer (data not shown).